Three-dimensional imaging of podocyte ultrastructure using FE-SEM and FIB-SEM tomography

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Human cardiac telocytes: 3D imaging by FIB-SEM tomography

Telocyte (TC) is a newly identified type of cell in the cardiac interstitium (www.telocytes.com). TCs are described by classical transmission electron microscopy as cells with very thin and long telopodes (Tps; cellular prolongations) having podoms (dilations) and podomers (very thin segments). TCs' three-dimensional (3D) morphology is still unknown. Cardiac TCs seem to be particularly involved...

متن کامل

Three-dimensional analysis of intermediate filament networks using SEM tomography.

We identified tomographic reconstruction of a scanning electron microscopy tilt series recording the secondary electron signal as a well-suited method to generate high-contrast three-dimensional data of intermediate filament (IF) networks in pancreatic cancer cells. Although the tilt series does not strictly conform to the projection requirement of tomographic reconstruction, this approach is p...

متن کامل

The SEM-FIB Workbench

The Nanorobotics series developed by Klocke Nanotechnik extend Scanning Electron or Ion Microscopes to material processing systems. The included automation allows the whole field of handling, assembly and manufacturing like known from a normal desktop system – now with nano-precision, including various kinds of image generation by using different sensors. Applications are in the field of Materi...

متن کامل

FIB-SEM imaging of carbon nanotubes in mouse lung tissue

Ultrastructural characterisation is important for understanding carbon nanotube (CNT) toxicity and how the CNTs interact with cells and tissues. The standard method for this involves using transmission electron microscopy (TEM). However, in particular, the sample preparation, using a microtome to cut thin sample sections for TEM, can be challenging for investigation of regions with agglomeratio...

متن کامل

Enhancing Materials and Device Analysis Capability in the SEM and FIB-SEM by using a Nanomanipulator

Nanomanipulators, whilst commonly used to lift out TEM samples prepared in the FIB, have a number of other potential applications and can aid in the chemical and crystallographic analysis of samples. Here, we discuss some of these alternative uses of nanomanipulators and show how they enable the EDS and EBSD analysis of thin samples in the FIB-SEM as well as serve as electrical probes and enabl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Cell and Tissue Research

سال: 2019

ISSN: 0302-766X,1432-0878

DOI: 10.1007/s00441-019-03118-3